LOCUS       40924_23409       12263 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB606 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 12263)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12263)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1891
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     protein_bind    1895..2019
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2056..2086
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2140..2796
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3141..3443
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3487..3611)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3649..3901
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3935..3951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3959..3975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3983..4013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4028..4049)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4085..4337)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4500..5048)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5100..5279)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5785..5809)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6330..7118
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7367..7955
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8141..8281)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8625..8819)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8888..9958)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10390..11016)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact cacggcatgg acgagctgta catcacaagt ttgtacaaaa aagctgaacg
     1921 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     1981 tacataatac tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca
     2041 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     2101 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     2161 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     2221 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     2281 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     2341 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     2401 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     2461 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     2521 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     2581 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     2641 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     2701 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     2761 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
     2821 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     2881 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     2941 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     3001 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     3061 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     3121 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     3181 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     3241 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     3301 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     3361 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     3421 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     3481 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     3541 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     3601 tacaaagtgg tgatgtacaa gtaaagcggc ccgagctcga atttccccga tcgttcaaac
     3661 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3721 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3781 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3841 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3901 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3961 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4021 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctattaat
     4081 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     4141 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     4201 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     4261 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     4321 caaatgtttg aacgatcggg gaaattcgag ctggggacgt ccccagctcg gtaccccctc
     4381 ctgggcacgg tcgtacgcga tccgccgcgg ttccacctca ccattgcggg tatgccagtt
     4441 gagttcgatg agttcccgta ccagctccaa ctcgggcgat gccacgcgca ctccgttcag
     4501 atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag
     4561 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg
     4621 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg
     4681 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag
     4741 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg
     4801 taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc
     4861 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc
     4921 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg
     4981 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct
     5041 gggctcatat cttattgccc cccgggatca gatctggatt gagagtgaat atgagactct
     5101 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     5161 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     5221 gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct
     5281 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     5341 ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     5401 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     5461 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
     5521 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     5581 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
     5641 tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc gagattaata
     5701 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     5761 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     5821 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     5881 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     5941 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     6001 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     6061 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6121 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     6181 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     6241 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     6301 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     6361 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     6421 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     6481 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     6541 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     6601 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     6661 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     6721 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     6781 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     6841 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     6901 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     6961 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7021 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7081 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     7141 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7201 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7261 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7321 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7381 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7441 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7501 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7561 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7621 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7681 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7741 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7801 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7861 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7921 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7981 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8041 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8101 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8161 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8221 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8281 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8341 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8401 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8461 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8521 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     8581 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8641 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8701 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     8761 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     8821 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     8881 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     8941 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9001 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9061 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9121 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9181 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9241 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9301 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9361 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9421 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9481 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9541 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9601 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9661 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9721 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     9781 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     9841 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     9901 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
     9961 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
    10021 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    10081 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    10141 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    10201 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    10261 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    10321 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    10381 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    10441 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    10501 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    10561 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    10621 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    10681 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    10741 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    10801 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    10861 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    10921 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    10981 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    11041 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    11101 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    11161 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    11221 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    11281 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    11341 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    11401 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    11461 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    11521 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    11581 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    11641 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    11701 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    11761 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    11821 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    11881 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    11941 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    12001 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    12061 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    12121 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    12181 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12241 caaatggacg aacggataaa cct
//
